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KMID : 0356219930240010134
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Journal of the Korean Military Medical Association
1993 Volume.24 No. 1 p.134 ~ p.146
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Histochemical Studyon the Change of Antidiuretic Hormone mRNA in Rat Hypothalamus Following Salt Loading
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Abstract
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1) Background:
@EN Antidiuretic hormone(ADH) is neurohypophyseal homone which is originally synthesized in the neuronal perikarya located at the supraoptic nucleus and paraventricular nucleus of hypothalamus and transported through their axons to
neurohypophysis.
ADH
increases body fluid by promoting water-reabsorption of kidney and also increases blood pressure by constricting blood vessels. Secretion of ADH is stimulated by hypertonic salt-loading and dehydration. But the change of ADH mRNA in hypothalamus
following salt-loading has not been fully understood. Therefore the study on the change of ADH mRNA with application of the technique of in situ hybridization using synthetic oligonucleotide was performed.
@ES 2) Method
@EN The rats are feeded with hypertonic NaC1 solution(2%)as drinking watr for 3 or 7 days. Oligonucleotide(27 mer)was synthesized for ADH mRNAs and labelled by the kination of radioactive 32P at 5' end. The labelled probe was applied to tissue
sections
for hybridization. The hybridization signal was detected by autoradiography using X-ray film and nuclear track emulsion. And the effect of salt loading on the expression of ADH mRNAs was studied with the application of the in situ hybridization
technique.
@ES 3) Results
@EN With salt loading, the size of magnocellular neurons in paraventricular nucleus and supraoptic nucleus increased and their cytoplasm became more basophilic, and the nucleoli were found more distinguished than control group. The hybridization
signal
for ADH mRNAs in magnocellular neurons of paraventricular nucleus increased by 2.5 and 3.5times in 3 days-and 7 days-loading group respectively. The signal in supraoptic nucleus increased by 2 and 2.5 times in 3 days-and 7 days-loading group
respectively.
@ES 4) Conclusions
@EN The results above indicate that the hypertrophy of ADH neurons and the increase of ADH mRNA by chronic salt-loading increase the synthesis and release of ADH, which in turn suppress the loss of fluid and electrolyte at kidney and play the
important
role in maintaining the osmotic pressure and homeostasis.
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KEYWORD
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